Immobilization of Glucose Oxidase on Glutathione Capped CdTe Quantum Dots for Bioenergy Generation

An efficient immobilization of Glucose oxidase (GOx) on an appropriate substrate is one the main challenges developing fuel cells that allow energy to be obtained from renewable substrates such as carbohydrates in physiological environments. The research importance biofuel relies their experimental robustness and high compatibility with biological organisms tissues or bloodstream aim obtaining electrical even living systems. In this work, we report use 5,10,15,20 tetrakis (1-methyl-4-pyridinium) porphyrin glutathione capped CdTe Quantum dots (GSH-CdTeQD) a support matrix for GOx carbon surfaces. Fluorescent GSH-CdTeQD particles were synthesized characterization by UV-Vis spectrophotometry showed particle size between 5–7 nm, which was confirmed DLS TEM measurements. Graphite Toray paper electrodes modified drop coating porphyrin, GOx, electrochemical activity toward glucose oxidation evaluated cyclic voltammetry, chronoamperometry impedance spectroscopy. Additionally, electrode explored scanning microscopy, finding near 70% surface covered active enzyme. sensitivity 0.58 ± 0.01 μA/mM apparent Michaelis constant 7.8 mM. addition BSA blocking protein maintained current response common interferent molecules ascorbic acid (AA) less than 5% interference percentage. Finally, complex employed anodes microfluidic cell (μBFC) order evaluate performance production. enzymatic used μBFC allowed us obtain density 7.53 mAcm−2 at maximum power 2.30 mWcm−2; open circuit potential 0.57 V observed cell. results suggest immobilize while preserving enzyme’s catalytic activity. reported arrangement viable option bioenergy production and/or quantification.